Protein transduction domain을 이용한 recombinant human bone morphogenetic protein-2 골재생효과
Bone regenerative effects of recombinant human bone morphogenetic protein-2 employed protein transduction domain
정성원, 김형준, 조규성, 김창성, 김남희, 육종인,
소속 상세정보
정성원 ( Jung Sung-Won ) - 연세대학교 치과대학 치주과학교실
김형준 ( Kim Hyung-Jun ) - 연세대학교 치과대학 구강악안면외과학교실
조규성 ( Cho Kyoo-Sung ) - 연세대학교 치과대학 치주과학교실
김창성 ( Kim Chang-Sung ) - 연세대학교 치과대학 치주과학교실
김남희 ( Kim Nam-Hee ) - 연세대학교 치과대학 구강병리학교실
육종인 ( Yook Jong-In ) - 연세대학교 치과대학 구강병리학교실
KMID : 0363020070370030497
Abstract
Bone morphogenetic proteins(BMPs) are regarded as members of the transforming growth superfamily with characteristic features in their amino acid sequences. A number of studies have demonstrated the biologic activities of BMPs, which include the induction of cartilage and bone formation. Recently there was a attempt to overcome a limitation of mass production, and economical efficieny of rh-BMPs. The method producing PTD by using bacteria have advantages of acquiry a mass of proteins. Hences, a new treatment which deliver protein employed by protein transduction domain(PTD) has been tried. The purpose of this study was to evaluate the bone regenerative effect of TATBMP-2 and TAT-HA2-BMP-2 employed by PTD from HlV-1 TAT protein for protein translocation in the rat calvarial model. An 8mm calvarial, critical size osteotomy defect was created in each of 32 male Spraque-Dawley rats(weight ). The animals were divided into 4 groups of 32 animals each (4 animals/group/healing interval). The defect was treated with TATBMP-2/ACS(Absorbable collagen sponge) (TATBMP-2 0.1mg/ml), TAT-HA2-BMP-2/ACS(TAT-HA2-BMP-2 0.1mg/ml), ACS alone or left untreated for surgical control(negative control). The rats were sacrificed at 2 or 8 weeks postsurgery, and the results were evaluated histologically. The results were as follows: New bone formation were not significantly greater in the TATBMP-2/ACS group relative to negative, and positive control groups. New bone was evident at the defect sites in TAT-HA2-BMP-2/ACS group relative to negative, positive control and TATBMP-2 groups. There were a little bone regeneration in TATBMP-2 groups. While, enhanced local bone formation were observed in TAT-HA2-BMP-2 group. But, The results was not the same in all rat defects. Therefore, further investigations are required to develop a method. which disperse homogenously, and adhere to target cells.
키워드
Bone regeneration;Bone morphogenetic protein;protein transduction domain
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